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KMID : 0360220200610040394
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Journal of the Korean Ophthalmological Society
2020 Volume.61 No. 4 p.394 ~ p.399
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Cytoprotective Effect of Rho Kinase Inhibitors against Oxidative Stress in Trabecular Meshwork Cells
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Kim Jae-Woo
Son Myung-Seo
Baek Min-Ju
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Abstract
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Purpose: We evaluated the protective effect of trabecular outflow drugs, Rho kinase (ROCK) inhibitors against oxidative stress in trabecular meshwork cells.
Methods: Primary-cultured human trabecular meshwork cells (HTMCs) were exposed to ROCK inhibitors at 10 and 20 μM Y-27632, ripasudil or fasudil for 24 hours, after pretreatment with 200 μM hydrogen peroxide for 30 min. The cell viabilities and metabolic activities were assessed using the Trypan Blue dye exclusion test and MTT assay, respectively. Reactive oxygen species (ROS) production was measured using the H2DCFDA assay, and the degree of apoptosis was measured with flow cytometry using annexin-propidium iodide double staining.
Results: In HTMCs, Y-27632 suppressed ROS production. Ripasudil and fasudil increased the metabolic activities and decreased the degrees of apoptosis. Fasudil showed the most cytoprotective effects among the three ROCK inhibitors tested.
Conclusions: Against oxidative stress, ROCK inhibitors decreased apoptosis accompanied by decreased ROS production, especially fasudil. ROCK inhibitors may therefore have cytoprotective properties in addition to increasing trabecular outflow.
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KEYWORD
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Apoptosis, Oxidative stress, Rho-associated kinase inhibitor, Trabecular meshwork
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